Proteases produzidas por bactérias da Amazônia com potencial fibrinolítico

Abstract

The bacteria, among other microorganisms, are natural sources of a variety of enzymes, including proteases of the fibrinolytic group. These enzymes are mainly responsible, catalyze the cleavage of peptide bonds of proteins, are widely used in various industries and biotechnological processes representing approximately 60% of the enzymes marketed worldwide. Proteases with fibrinolytic activity are capable of lysing fibrin preventing thrombotic diseases or corrals for the degradation of blood clots. These enzymes are gaining importance in medical, pharmaceutical and food due to the effectiveness of fibrinolytic action and the possible application of the same in the treatment of cardiovascular disorders. In this work thirty colonies of bacteria of the genus Bacillus, isolated from soil sample contaminated with oil in the Amazon. Were studied for their potential proteolytic and fibrinolityc. In the analysis of the potential fibrinolytic prevailed Bacillus subtilis (21%) and Bacillus circulans (13%). The thirty isolates were subjected to submerged fermentation for 24 hours, and the enzymatic extract produced was performed to determine the proteolytic activity and fibrinolytic activity. The highest value obtained for the proteolytic activity was 7.073 U / mL, and fibrinolytic activity occurred in the formation of halos in Bacillus fibrin plate B 16. The results demonstrated the potential of producing proteases. A fibrinolytic activity was shown in Bacillus B16, selected for the characterization of proteases expressed that activity in a wide pH range and at all temperatures tested (25oC to 80oC), showing optimal activity at pH 8.4 and 45oC, respectively.