Estudo químico de fungos endofíticos associados a Duguetia stelechantha e Rollinia sp. (Annonaceae)

Abstract

The plants of the genera Duguetia and Rollinia belonging to the family Anonnaceae have several reports on its composition mainly by the presence of alkaloids and acetogenins. These compounds have proven biological activity as antiparasitic, antitumor, insecticides and antibacterial. However, there is little information about the plant endophytic microorganisms of these genres. In the present work it was presented the chemical study of two strains of endophytic microorganisms from Duguetia stelechantha, and a strain isolated from Rollinia sp. based on results of antimicrobial assays of the extracts on a pilot scale against Candida albicans, Penicillium avellani, Bacillus cereus and cytotoxic assays against three tumor cell lines. Based on the results of these tests were selected strains DgCr22.1b (result> 90% against tumor cell lines) and DgC32.2 (best result against B. cereus) (Duguetia stelechantha) and AnspCr11.1 (Rollinia sp.) for large scale cultivation and chemical study. Were obtained two substances isolated from a strain DgCr22.1b being a polyketide and a xanthone. Strain DgC32.2 five substances have been isolated and identified two of three dyes belonging to class azaphilone and a steroid. Were isolated and identified three substances of lineage AnspCr11.1 which are an isocoumarin, a chromone and an alkenoic acid known as cordyanhydride B.