Detecção de Bunyavírus em flebotomíneos coletados em duas áreas do estado do Amazonas, Brasil

Abstract

The arbovirus are virus group with a complex life cycle, once vertebrate hosts as well as arthropod vectors are involved. The Phlebovirus are arboviruses of the Bunyaviridae family transmitted mainly by Lutzomyia insects in the new world and Phlebotomus in the old world. These insects are important vectors of tropical diseases, such as leishamaniasis, causing outbreaks of febrile syndromes worldwide. A large fauna of phlebotomus is observed in the Amazon region and a few studies aimed to detect virus at those vectors. The present study aimed to the detection of arboviruses in phlebotomus collected in the Reserva Florestal Adolpho Ducke, Manaus and at the Ramal Nova Esperança, Manacapuru, Amazonas. The phlebotomus were collected using adapted CDC light traps, including parallel collections with manual aspirators. Speciemens were grouped in pools by sex and trap, and subsequently macerated. The macerated samples were inoculated into VERO cells, following by indirect immunofluorescence. The positives samples by flow cytometry were submitted to molecular assays, in order to amplify segments S and L of the Bunyavirus. Among the collected pools, 17 out of 243 were positive by flow cytometry, those samples presented displacement of positive cells within a range 1.19 – 71.6%. Also, molecular methods were applied for detection of Bunyavirus, following capillary and next generation sequencing. However, no virus was identified with this techniques. The samples are stored for futures studies, in order elucidate questions related to the presence of medical important arbovirus infecting phlebotomus at the Brazilian Western Amazon.