Avaliação histopatológica do efeito de suspensões de própolis na doença periodontal inflamatória induzida em ratos.

Abstract

The Periodontal disease (DP) is very strongly associated with the dental biofilm and may evolve the formation of a periodontal pocket. The propolis has been used as a medicinal product since 300 b.C, and has the following characteristics: anti-cancer activities, antioxidant, anti-inflammatory, antimicrobial, and antifungal. The propolis antimicrobial activity in face of periodontalpatogens has been investigated though in vitro and in vivo tests, mainly with the etanolic extracts. The following paper had as an objective evaluate the histological response of the inflammatory DP induced on the rats periodontal tissue after the use of a watery suspension of propolis (SAP), at 0,1%, 1%, and 10%, for subgingival irrigation. Was used 84 wistar rats (Rattus novergicus), weighting between 210 and 290g with eight weeks old. They were distributed in the following experimental groups: group I (n= 14, SAP at 0,1%), group II (n=14, SAP at 1%), group III (n=14, SAP at 10%), group IV (n=14, Tween`s solution 80 at 5%), group V (positive control, n=14, with induced DP and without treatment), group VII (n=14, chlorhexidine at 2%) and group VI (n=84, negative control, not induced contralateral teeth and not treated on animals of groups I, II, III, IV, V e VII). The inflammatory DP was induced with the technique that uses the ligation with cotton yarn during a period of 15 days on the cervical of the first left inferior molar. After the removal of the yarn the periodontal pocket was irrigated 3 times (1st, 4th and 7th days) with the tests solution and the sacrifice was done on the 15th and 30th day after treatment. Was done the test exact of Fisher (p-value 0,05). Analyzing comparatively the results you may suggest that the propolis suspension on the concentration of 0,1% is efficient considering the short term inflammatory response (15 days), associated to the presence of dense gingival fibers, blood vessels without congestion, no dental reabsorption and bone loss. There for may be used as an alternative treatment for the periodontal disease, needing further studies to demonstrate the biological viability on the use as supporting on the periodontal therapeutic.