Utilização de peptídeos quiméricos das proteínas de envelope (E) , não estrutural 1 (NS1) e não estrutural 3 (NS3) para produção de insumos diagnósticos da dengue

Abstract

Dengue infection has become a public health problem in tropical and sub-tropical areas. It is a viral disease transmitted by arthropods with high mortality rates. Its clinical diagnosis requires laboratory confirmation because several other conditions can also cause similar symptoms with acute fevers as well as the primary use antibodies or antigens for detection of DENV. The continued development of diagnostic tests that are cheap, sensitive, specific, easy to perform, and that are capable of giving early diagnosis of the dengue virus infection is still a needed. Using bioinformatics, we mapped peptides of Envelope protein (E), Nonstructural 1 and 2 which have antiginity, hidrofility and probality to apper on the surface. The first peptide, with molecular weight of the 17KDa, was called E/ENS3 and corresponds to two Envelope protein (E) sequences and a Nonstructural 3 sequence. The second peptide, with molecular weight of the 15Kda, was called ENS1/3 and it corresponds to a Envelope protein (E) sequence, a Nonstructural 1 sequence and Nonstructural 3 sequence. The peptides were expressed in prokaryotic sistem, purified and used in ELISA (Enzyme-Linked Assay Imunoabsorvente). Using Indirect ELISA in the IgM and IgG antibody detection previously characterized, we obtained as results: (1) IgG research using E/ENS3 and ENS1/3 peptides, sensibility was 65% and the specificity was 91,7% and (2) IgM research, sensibility was 75,7% and and the specificity was 91,8%. These results indicate that use of both E/ENS3 and ENS1/3 peptides may be a alternative to the serological diagnosis of dengue.