Identificação de biomarcadores lipídicos em tumores mamários e correlação com IL-17 como fator inflamatório

Abstract

Numerous molecules of the tumor microenvironment can act on the progression of breast cancer. IL-17 and lipid species have been identified as protumorigenic factors involved in proliferation, cell migration and metastasis. In this context, the aim of this study was to quantify lipid species in breast tumors, evaluate the influence of IL-17 on lipid metabolism and identify potential biomarkers. In this study we demonstrated that IL-17 and EICs levels were increased in breast tumors. We also show that the metabolism of sphingolipids is altered in breast cancer with an increase in Cer, dH and LacCer and a decrease in SM in the tumor tissue and the distribution of these lipids is different in cancer subtypes with predominance of SM in the luminals A and B and dHCer in HER-2. In addition, we observed distinct profiles of sphingolipids in pre- and post-surgical sera, which the distribution of these lipids after tumor removal presented a profile similar to controls, except for HER-2. We found that there was a correlation between IL-17 and sphingolipids, inferring that cytokine can regulate the metabolism of these lipids. Multivariate analyzes revealed that TXB2, 11-HETE, SM C16:0 and C18:1 are potential biomarkers for breast cancer regardless of tumor subtype and dHCers d20:0/20:0 and d18:0/24: 0 are potential biomarkers for luminal B and HER-2 subtypes, respectively. IL-17 also showed biomarker potential for luminal B. These results suggest that lipid mediators are potential candidate biomarkers to support in the diagnosis and monitoring of disease progression.