Uso da creatina na alimentação in ovo

Abstract

This study evaluated the effects of in ovo feeding of creatine on hatchability, hatching weight, embryo mortality, thermodynamic yields, and organ developments in Rhode Island Red chicks. A total of 427 fertile eggs with viable embryos was randomly distributed to 7 treatments of 61 replicate eggs. The experimental groups were composed of untreated control (control), a sterile buffered solution (0.5% saline), and solutions containing increased levels of creatine (0.5, 1.0, 1.5, 2.0, and 2.5%). On d 16 of incubation, eggs were injected with solutions containing creatine dissolved in 500 μL of 0.5% sterile saline. After injection, the pinholes in the eggs were sealed with molten paraffin and moved to a hatching machine. Immediately post hatch, were evaluated the chicks’ weight, hatchability, thermodynamic yields, and embryo mortality. Five able chicks of each treatment were randomly selected and slaughtered by cervical dislocation to evaluation the heart, and gastrointestinal organ and region developments. In ovo feeding of creatine directly affect the hatching characteristics of injected eggs. The in ovo feeding of creatine to 16-d-old embryos directly influence hatchability and embryo mortality, especially in the first hours post-injection. Increased levels of creatine provide a linear increase in chick weight, development of gastrointestinal tract, and thermodynamic yields.