Susceptibilidade de Mycoplasma Hominis e Ureaplasma Sp. a antimicrobianos

Abstract

Mycoplasmas are microorganisms lacking cell walls with reduced ability Biosintética, which makes them extremely fastidious growth. The strength of these microorganisms to antimicrobials used in his therapy as tetracyclines, macrolides and quinolones have been reported with increased frequency. The determination of antimicrobial susceptibility is particularly difficult because not shown in broth turbidity which complicates the standardization of the inoculum and are extremely susceptible pH conditions. The method most commonly used for this purpose is based on metabolic inhibition using specific substrates. This study evaluated the susceptibility of isolates of Mycoplasma hominis and Ureaplasma sp. stored in the period from 2011 to 2012. The methodology used for the isolation comprised culture techniques, for storage and freezing of the strains we used selective broth enriched PPLO and BHI, Thawing of isolates was succeeded subculture in broth enriched selective and differential agar A7 for phenotypic characterization. Quantification, identification and antimicrobial susceptibility testing was performed using the the triage system Mycofast Evolution ® Screening (Elitech Group), whose identification is based on bacterial susceptibility by Identibiotiqué system. The susceptibility of the isolates was determined against antimicrobial agents Doxiciclina 8 μg/ml, Roxitromicina 4 μg/ml and Ofloxacina 4 μg/ml, widely used in the empirical treatment of patients with urogenital tract infections. Our study showed high level of resistance of the isolates to doxicycline and ofloxacin. This is the first study involving isolation and susceptibility profile of these agents undertaken in the Amazonia.