Uso do extrato líquido do camu-camu (Myrciaria dubia) com adição de lecitina como meio diluidor de sêmen de galo

Abstract

The objective of this study was to evaluate the potential of the liquid extract of Camu-Camu (Myrciaria dubia), in conjunction with soy lecithin as a natural dilution medium for rooster semen at room temperature, as well as its preservative effect on membrane integrity plasma of sperm. The experiment was carried out in the Poultry Sector with the Laboratory of Animal Anatomy and Physiology of the Federal University of Amazonas. Fifteen breeding roosters (Gallus gallus) of the FC Cabocla 1 strain, at 40 weeks of age, were fed with a diet calculated according to nutritional requirements and previously conditioned to release semen by mechanical action. The ejaculate was diluted in Camu-camu Liquid Extract and Soy Lecithin (ELCL) and in Beltsville Poultry Sêmen Extender-BPSE®,, both following a 1:2 ratio (semen: extender). The storage time for in vitro semen analysis, already homogenized with the extenders, was divided into 15 min, 30 min, 45 min and 60 min. Ejaculate samples of all items were analyzed for macro and microscopic aspects through physical (motility, sperm vigor, volume, pH, and concentration) and morphological (major, minor and total sperm defects) assessments. Sperm motility of semen maintained in the presence of the commercial extender did not change (p > 0.05) over time. In in natura semen, motility decreased (p ≤ 0.05) after 45 min. In semen maintained with Camu-camu+lecithin, motility was maintained (p > 0.05) up to 45 min. Progressive rectilinear motility did not change (p > 0.05) over time of storage in semen containing commercial extender. Using ELCL, progressive motility was maintained (p > 0.05) up to 30 min. In the first 15 minutes, the amount of total sperm pathologies was similar (p > 0.05) between treatments. However, at 30, 45 and 60 min, the total amount of pathologies was higher (p < 0.05) in semen containing ELCL. The percentage of rooster spermatozoa with total morphological defects was lower (p< 0.05) in semen containing commercial extender when compared to in natura semen and extender containing Camu-camu + lecithin. Similar values were observed in the larger morphological defects. The percentage of isolated and straight heads were not affected by the extenders (p >0.05). The percentage of spermatozoa with a strongly bent tail and a bent tail was lower (p < 0.05) in the semen containing commercial extender when compared to the other treatments. That said, the Camu-camu liquid extract in conjunction with soy lecithin did not obtain satisfactory results as semen preservatives at room temperature. However, further research with variants that have not been studied can give a new narrative to this process, since the properties contained in this fruit and in lecithin are necessary for the spermsurvival process.