Produção de álcool a partir de amido utilizando-se amilases recombinante

Abstract

There some decades the search for alternative energetic sources is mundialy on the focus. For environmental reasons economic and geopolitical this search has been strained and one of the sources of alternative energy that awakens the trend to replace fossil fuels, is the ethanol that is a cleaner fuel. Even more, companies, governments and researchers has been focused their reachers to it. The obtained alcohol by biomass fermentation is called "bioethanol". Brazil's position about the full use of biomass is very privileged because of its greatest world’s biodiversity, an intense solar radiation, abundance water, climate’s diversity and biomass from biomass products on a large scale, Brazil presents itself as the most advanced country in this technology once it is the currently largest bioethanol producer in the world. In this context the goal of this study is presenting the ethanol’s production viability from a efficiently hydrolyzate of cassava starch Manhiot esculenta Crantz which was obtained with the recombinant amylases (α-amylase and glucoamylase), resulting from the previous work developed in the research group by OLIVEIRA (2009) and CARMO (2010). The hydrolyzate was fermented by the yeast Saccharomyces cerevisiae commercial yeast on systems known as fermentometers. The hydrolyzate resulted in a 20 gL-1 ethanol concentration in 6 h, with hydrolysis efficiency of exceeding 57% volumetric productivity 3.33 g/L-1h-1 and yield factor of substrate consumption of 0.29 gg-1.