Silagem de milho grão reidratado submetido a níveis crescentes de pepsina aberto em diferentes tempos

Abstract

Corn starch digestibility is related to the grain's prolamine content. Corn produced in Brazil has flint endosperm. The ensiling process and the use of proteases can hydrolyze prolamines making starch more digestible. Thus, the objective of this work was to evaluate increasing levels of addition of protein-degrading enzymes (proteases) in ground corn rehydrated and ensiled for different times under starch digestibility. For the development of the study, ground dry corn was rehydrated at a moisture content of 40%, added pepsin (Pepsin 1:10000) in proportions of 0, 25, 50, 100 and 500g/ton of corn in dry matter and ensiled by 0, 15, 30, 60, 120 and 240 days in experimental silos of special plastic bags and vacuum closed, with a capacity of approximately 500 g of silage. The analyzed variables were dry matter (DM), pH, ammonia nitrogen content (N Ammoniacal), crude protein content (CP), starch content and zein content (prolamine). The samples showed positive results in relation to the ensiling time, which increased the ruminal digestibility of dry matter for 7 hours, reducing the zein protein levels with a consequent increase in the ammoniacal nitrogen contents. Furthermore, the results also showed that the addition of 50 grams of pepsin per ton of silage increased ruminal digestibility for 15 days of silage.