Produção de hidrolisado proteico de resíduo de pirarucu (Arapaima gigas)

Abstract

The processing of arapaima (Arapaima gigas) generates a lot of waste that can be reused for development of new products of industrial interest. The aim of this study was to evaluate the production of protein hydrolyzate from pirarucu waste using pancreatin and Aspergillus flavofurcatis proteases. The raw material was minced fish of arapaima originated from Mamirauá Sustainable Development Reserve, State of Amazon. The process was conducted under constant agitation and controlled time and temperature. The minced fish and the hydrolysates were analyzed for chemical composition and microbiological quality. From these experiments two products were developed: an pirarucu protein hydrolyzate obtained of commercial enzyme (HPPEC) and another one prepared with microbial enzyme (HPPEM). The hydrolysates were analyzed for degree of hydrolysis, digestibility and amino acids profile. The results showed that the protein content in HPPEC (71.30 %) was significantly higher than HPPEM (57.26 %), however, in both products, were observed high values of digestibility, absence of microbial contaminants and reduction of lipid content. The aminogram showed the presence of all essential amino acids in both products. Among the enzymes used, pancreatin had higher hydrolytic efficiency, but the higher levels of digestibility, hydroxyproline content and taurine were deterrmined in the final product developed with A. flavo-furcatis enzymes