Libidibia ferrea L.: Avaliação in vitro da ação antimicrobiana e citotoxicidade de extratos e de uma formulação em orabase.

Abstract

The aim of this study was to evaluate the antimicrobial activity of aqueous extracts of the fruits and stem bark and a formulation orabase of Libidibia ferrea against biofilm microorganisms by agar diffusion method and broth microdilution, and to evaluate toxicity through hemolysis assay and fibroblast cell culture. For this purpose, aqueous extracts were prepared and from these was designed one orabase. The microorganisms used for determination of MICs were Streptococcus salivarius (ATCC 7073), Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557), Lactobacillus casei (ATCC 7469) and Candida albicans (40040 INCQS). As a positive control Chlorhexidine 0.12% and as a negative control the vehicle. In cytotoxicity tests the extract of the stem bark, the formulation vehicle, adjuvants and Orabase formulation were tested. In hemolytic activity, as a positive control Triton X-100, and in the activity of cultured cells doxorubicin, drug pattern of cell viability. As negative controls, the carrier and the diluent of the formulation. The results were presented using descriptive statistics. The results showed that both extracts of the fruits as the stem bark showed antimicrobial activity. MICs for the stem bark extract of jucá were 2.62 mg/mL (Streptococcus mutans), 2.25 mg/mL (Streptococcus oralis), 4.12 mg/mL (Lactobacillus casei), 2.62 mg/mL (Candida albicans) and 3.0 mg/mL (Streptococcus salivarius). As for the extract of the fruit MIC’s values were 3.75 mg/mL (Streptococcus mutans), and 3.37 mg/mL (Streptococcus oralis , Candida albicans and Lactobacillus casei). There was no activity for Streptococcus salivarius. Regarding the Orabase formulation MICs were 0.45 mg/mL (Streptococcus mutans), 0.48 mg/mL (Streptococcus oralis), 0.41 mg/ mL (Lactobacillus casei), 0.48 mg/mL (Candida albicans) and 0.48 mg/mL (Streptococcus salivarius ). As for the hemolysis test, both the extract of the stem bark as the formulation and its components did not cause hemolysis in mg / mL. There was an absence of hemoglobin in the supernatant and sedimented erythrocytes. The solutions tested showed no toxicity when tested against fibroblast cells. Based on the results it was concluded that the extract of the stem bark and the orabase formulation of Libidibia ferrea L. showed antimicrobial activity against microorganisms of the biofilm and were not toxic when tested in erythrocytes and cell culture.