Análise de alcaloides indólicos antitumorais produzido por Penicillium chrysogenum guiada por Espectrometria de massas e Molecular Networking

Abstract

Endophytic fungi represent an abundant source of chemically bioactive substances, especially the cytotoxic action that has been encouraging research in several areas. Thus, the present study consisted of identifying indolic alkaloids from extracts of the fungus Penicillium chrysogenum in different culture media by mass spectrometry (MS) and by molecular network Networking (MN), as well as assessing the in vitro anticancer potential of its extracts. Through the MS and MN analyzes, it was possible to identify 16 substances, eight indole alkaloids: (1) glandicoline A, (2) glandicoline B, (3) oxaline, (4) meleagrine, (5) roquefortina C, (6) roquefortina D, (7) neoxaline, (8) tryptophan), two pyroglutamyl peptides (9) pyroglutamylphenylalanine, (10) pyroglutamylvaline, and six piperazine alkaloids: (11) (L proline-L-valine) cycle, (12) cycle (L-leucine-L-proline), (13) cycle (L-phenylalanine-4 hydroxy-proline), (14) cycle L-valine-L leucine, (15) cycle (L-phenylalanine-D- Proline),(16) cycle (phenylamine-leucine). It is worth mentioning that there is no record in the literature about the production of substances 9, 10 and 13, 14, 15, and 16 about the species P. chrysogenum. Thus, these substances identified by LC MS / MS and the MN network are unprecedented contributions to the species under study. The extracts prepared in different culture media did not show a promising cytotoxic potential on the tested cells. Through screanning, the extract prepared in ISP2 medium showed 63.9% and 20.1% for tumor cells HepG2 (human hepatocellular carcinoma) and B16-F10 (murine melanoma), respectively. Thus, it is possible to consider that the studied fungal species produced alkaloids (indole and piperazine type) and peptides, some of which are responsible for cytotoxic activity (according to the literature). Thus, the importance of continuing studies with this fungal strain on phytochemical isolation and pharmacological activities.