Micropropagação e Aclimatização de Camapú (Physalis angulata L.).

Abstract

Camapú (Physalis angulata L), belonging belongs to the Solanaceae family, is an annual herb of comospolite origin that stands out for presenting excellent agronomy and pharmaceuticals. As well as most of the medicine species camapú comes being collected through extrative processes and the harvested vegetal material is heterogeneous and low quality. The present study proposes to establish an efficient methodology of micropropagation in vitro and for commercial production of changes. Camapú segments nodal contend untill two axillary egg yolks and with 1 till 1,5 cm of length had been restrained from plants kept in vitro and inoculated in MS medium, with pH adjusted for 5,7 before the inclusion of 0,7% aga dissolved by heating. The plantlets adventitious sprouts of explants inoculated MS medium increased 3% of sucrose and supplemented with different concentrations of BAP (0; 0,5; 1,5; 2,5;5,0 mg.L-1) and IAA (0; 0,5; 1,5; 3,0 mg L-1), had better presented development in MS medium exempt IAA and BAP. It was verified that the plantlets adventitious sprouts of explantes inoculated in MS medium modified in the inorganic nitrogen concentrations (0; ¼; ½; 1 e 2 MS) and sucrose (0; 7,5; 15; 30 e 45 g.L-1) had not presented significant differences in the concentrations ½ MS and 1MS. In the acclimatization phase in greehouse with intermittent nebulizacion and 50% of shady, the substrates contend the mixture from vermiculite + florest soil (1: 1 v/v) promoted a bigger index of survival and development of the plants.