Contribuição ao estudo químico e farmacológico de espécies vegetais da Amazônia contra o câncer

Abstract

This study aimed to analyze the cytotoxic and antioxidant extracts obtained from 20 plant species in the Amazon. A relationship was shown between Artemia salina mortality with antioxidant activity and cytotoxicity in mouth cancer cells and antioxidant activity by chemical and biological method. Furthermore, it was found cytotoxicity of samples of breast cancer cells, fibroblasts and macrophages. The botanical material was from the Adolpho Ducke Forest Reserve, involving roots, stems and leaves. The most active extracts the high polarity and its fractionation was performed with Sephadex LH20 eluting with methanol plus 50% water in the last two fractions. The methanol extract of Inga cordatolata leaves was the most active antioxidant in tests on chemical and cellular level. Showed higher cytotoxic effect to other samples both in the crude extract, as in its fractions. In spite of the high cytotoxicity observed in mouth cancer cells, this species does not induce killing of macrophages. In small concentrations of this antioxidant extract has been shown, whereas in higher concentrations showed significant cytotoxicity. Chemical analysis by High Performance Liquid Chromatography and Liquid Chromatography Ultra Efficiency suggests the presence of quercetin and isorhamnetin 3-O-glucoside on their constituents. It was concluded that methanol extract of the leaves has Inga cordatolata, associated form, the biological properties investigated in this work. Among all samples, it presents more likely to turn out to be a source of anticancer drugs. However, it is not possible to state that these effects are maintained in vivo. Thus, it is suggested the preparation of nanocapsules to see if there would be selective drug distribution between normal and cancerous tissues, and the activity of this sample would be different in each tissue