Estudo da microbiota da rizosfera do guaranazeiro (Paullinia cupana Kunth var.sorbilis (Mart.) Ducke) pelo sequenciamento do gene 16S rDNA

Abstract

The production of guarana in the Amazon region faces unfavorable phytosanitary conditions due to the presence of anthracnose, a disease caused by Colletotrichum spp. Taking into account the existing dynamics in plant-microorganism interaction took place this study to identify and verify the wealth and bacterial diversity associated with the rhizosphere of guarana with and without symptoms of antracnose. April 2011 were collected at the Fazenda da AMBEV located in Maués-AM district, guarana the rhizospheres with and without symptoms of antracnose. To extraction of genomic total DNA of bacteria uncultured present in the rhizosphere of guarana with symptoms of the disease and asymptomatic. The bacteria were identified by sequencing the 16S rDNA gene using as molecular tool the pyrosequencing and bioinformatics resources. The results indicate that the rhizosphere Sound guarana is richer and diverse in relation to the patient guarana. The predominant Filo was the Bacteroidetes in both physiological conditions of guarana, followed by Proteobacteria, Actinobacteria and Acidobactérias. The predominant genera accessed in the rhizosphere of the patient guarana were Chryseobacterium, Flavobacterium, Pedobacter, Sphingobacterium and healthy plants were represented by Rhizomicrobium, Acidicaldus, Sphingomonas and Rhodoplanes.Os common genres will rhizosphere of sick and healthy guarana were represented by Pseudomonas, Taibaiella, Mucilaginibacter, Candidatus Koribacter, Granulicella and Acidothermus.The presence of Colletotrichum spp appears to influence the composition of the microbiota rhizosphere of guarana.