Extração de compostos bioativos de interesse comercial a partir do cultivo da microalga Spirulina platensis em meio contendo subprodutos agroindustriais da Amazônia

Abstract

Spirulina platensis, a widely studied microalgae, has been successfully cultivated using agro-industrial by-products as an alternative source of growth, offering a sustainable approach to its large-scale production. Its cultivation cycle involves several steps, including inoculation, growth, harvesting and processing. During the cultivation cycle, the production of phycocyanin, a natural pigment with antioxidant properties, is closely monitored. To extract phycocyanin, solvents are commonly used, followed by purification processes. Studies have investigated the stability of phycocyanin and its antioxidant activity, providing insights into its potential use in pharmaceutical, cosmetic and food applications. These advances highlight the potential of Spirulina platensis as a valuable source of bioactive compounds and its role in promoting sustainability and health. Therefore, the objective of this work is to evaluate the insertion of an agro-industrial byproduct from the Amazon and test its effectiveness as an alternative source of growth, in addition to evaluating the productivity of compounds of interest during cultivation cycles. The compound of interest will then be extracted in different solvents in order to evaluate its stability and antioxidant activity in different environments. The results obtained demonstrated that the byproduct was effective and that the concentration of Spirulina platensis biomass was higher when compared to the synthetic medium, the proximal composition data showed that the value of biomass proteins in the alternative medium was higher with a value of 8,63% compared to the biomass of the synthetic medium, which was 4,95%. And saturated fatty acids such as palmitic acid were superior to the synthetic medium, as well as unsaturated fatty acids that showed higher or similar values. The optimum harvest point for determining phycocyanin productivity was obtained in the T4 cycle (12 days) and for extraction, distilled water was the one that showed the greatest efficiency in relation to antioxidant activity, for stability buffer solutions pH 6,0 and 7,0 were the ones that showed the best results. We conclude from the results obtained that the insertion of the byproduct proved to be effective for the production of biomass and phycocyanin and can be used for large-scale production, in order to increase the production of biomass from the microalgae Spirulina platensis.