Genotipagem de Cryptococcus neoformans e C. gattii isolados de poeira domiciliar e avaliação da susceptibilidade a antifúngicos e da presença do antígeno em moradores de uma comunidade rural do Amazonas

Abstract

Cryptococcosis is a systemic mycosis caused by the inhalation of resected propagules of Cryptococcus fungus. The two main species that cause disease are Cryptococcus neoformans and Cryptococcus gattii. In this study the objective was to evaluate the presence of cryptococcosis agents in residences of a rural community of Amazonas and characterize the environmental isolates of C. neoformans and C. gattii for genetic diversity using multilocus sequencing typing (MLST) and antifungal susceptibility using E-test. The total of 103 samples of indoor dust were analyzed, in 12 houses the isolation of Cryptococcus neoformans and / or C. gattii molecular types VNI and VGII were possible. The STs knowed for C. gattii ST46 and ST93 for C. neoformans were found. In addition, new alleles were found, resulting in new STs for the two species. The evaluation of the antifungal susceptibility revealed the presence of two strains of C. gattii with fluconazole resistance. All residents had negative results CrAg, despite the dust of their homes present cryptococcosis agents.