Detecção do vírus dengue em mosquitos Aedes Aegypti (linnaeus, 1762) (diptera: culicidae) e aedes albopictus (skuse 1894) (diptera: culicidae) capturados na zona urbana da cidade de Manaus, Amazonas

Abstract

The study had for objective the detection and typing of the dengue virus, in the vectors Aedes aegypti and Aedes albopictus through the Polimerase Chain Reaction (PCR) using specific primers. During the period of December of 2005 to December of 2006 8984 mosquitoes were collected, of these 827 they were Aedes, being 819 Ae. aegypti (414 females and 405 males) and 8 Ae. albopictus (7 females and 1 male), in 46 neighborhoods of the city of Manaus including all of the geographical areas of the city. The females were grouped in pools from 1 to 10 mosquitoes for microtubes, in agreement with the gender, it dates from collection, neighborhood and stocked to -70C in the same day of the collection, totaling 143 pools (138 of Ae. aegypti and 5 of Ae. albopictus). Those pools were macerated in PBS containing albumin, and a bracket of the softened was removed for inoculation in cell culture, and other bracket was used to accomplish the extraction of the viral RNA. Once extracted, RNA was submitted the reaction of reverse transcription following by the chain reaction of the polimerase (RT-PCR). The positive samples in RT-PCR were submitted the reaction of semi nested PCR and the products of this reaction were resolved in agarose gel to 1,5% with etídio bromide. Of the 143 analyzed pools, 114 (111 of Ae. aegypti and 3 of Ae. albopictus) they were positive for DENV 3, just a pool was shown positive for two sorotipos, DENV 1 and DENV 3. The prevalence pools Ae. aegypti infected with DENV 3 in the city of Manaus was of 53%, being larger in the areas Center-west (70%), South (60%) and West (53%), and smaller in the area East (23%). This is the first report of the detection of DENV 3 in field-caught Ae. albopictus naturally infected in Brazil. The monitoring of the circulation viral in mosquitoes with the use of the technique of RT-PCR allows the previous knowledge of the levels of spread viral in certain areas contributing to determine the time and place to apply the prevention measures and control.