Investigação química e busca de atividade antifúngica em resíduos madeireiros de Protium puncticulatum Macbr

Abstract

Traditional logging activities in Brazil are waste generators and much of it is underutilized due to lack of knowledge about their use. In partnership work, the research group "Plants of the Amazon: Chemistry, Chemosystematics and Biological Activity", use as wood lefovers from the Wood Technology Laboratory (LTM) of the National Institute of Amazonian Research (INPA) as a research source, to provide the scientific community with chemical and biological knowledge about the wood of different species. In this study the remains of the wood of Protium puncticulatum (Burseraceae) were available and used to chemical research and evaluation of antifungal activity. After the chromatographic fractionations and the analysis by Nuclear Magnetic Resonance (NMR) spectroscopy, the work with the hexane extract resulted in the mixture of triterpenes α and β-amirina, in addition to lupeol; of steroids β-sitosterol and stigmasterol. The fractionation with methanolic extract led to the isolation of the lignans 7-oxo-parabenzolactone and 7'-hydroxy-9α-methylcubebin. Considering the high biological potential of the compounds belonging to the class of lignans, the antifungal test was carried out with the lignans isolated from the wood residues against the strains of Cryptococcus neoformans (ATCC90112), Cryptococcus gattii (ATCC32269) and Candida albicans (ATCC60193). The lignan 7'-hydroxy-9α-methylcubebin showed higher activity against the strains of C. neoformans and C. gattii (MIC 160 μg/ml) by broth microdilution technique.