Perfil alcaloídico de Anonáceas do campus da UFAM e estudo fitoquímico e biológico de Ephedranthus amazonicus R. E. Fries (Annonaceae)

Abstract

This paper presents the results of studies of the profile alkaloids in thin layer chromatography (TLC), the species Xylopia aromatica, X. excellens, X. benthamii, Annona sericea, A. ambotay, Anaxagorea brevipes, rollinia exsucca, Duguetia ulei and Ephedranthus amazonicus (Annonaceae), found in the vicinity of the Campus of the Amazon and the phytochemical and biological species Ephedranthus amazonicus R. E. Fries, Annonaceae plant belonging to the family that is distributed throughout the Amazon region is known as “envira-dura”. For the chromatographic profile of the alkaloid fractions of the species studied can be observed the presence of oxoaporphine alkaloids liriodenine and O-metylmoschatoline, regarded as one of the markers of the family Annonaceae. Preliminar analysis by FIA-ESI-IT-MS of alkaloid fractions from leaves and twigs of E. anazonicus indicated the presence of alkaloids O-metilmoschatoline and annamontine leaves, liriodenine and lisicamine in twigs, isomoschatoline and 9-metoxy-isomoschatoline in both parts of the plant, in agree with the TLC data. Other MS/MS and LC/MS analyses are necessaries for the validation of these results. Phytochemical study of methanolic extract of leaves and twigs of Ephedranthus amazonicus led to the isolation of oxoaporphine alkaloids O-metylmoschatoline, liriodenine and isomoschatoline. The identification of isolated compounds was performed by means of spectroscopic analysis (1H and 13C 1D and 2D) and comparison with literature data. The extracts and fractions of E. amazonicus tests were done to evaluate its biological activity. In cytotoxicity test on Artemia salina all extracts and fractions showed themselves active. The methanol extacts, the alkaloid fractions and isolated compounds showed antioxidant active by testing ORACFL.